Accession PRJCA017467
Title A Cytological Atlas of the Human Liver Proteome from PROTEOMESKY-LIVERHu 2.0, a Publicly Available Database
Relevance Medical
Data types Map
Organisms Homo sapiens
Description The liver plays a unique role as a metabolic center of the body, and also performs other important functions such as detoxification and immune response. Here, we establish a cell type-resolved healthy human liver proteome including hepatocytes (HCs), hepatic stellate cells (HSCs), Kupffer cells (KCs), and liver sinusoidal endothelial cells (LSECs) by high-resolution mass spectrometry. Overall, we quantify total 8354 proteins for four cell types and over 6000 proteins for each cell type. Analysis of this data set and regulatory pathway reveals the cellular labor division in the human liver follows the pattern that parenchymal cells make the main components of pathways, but nonparenchymal cells trigger these pathways. Human liver cells show some novel molecular features: HCs maintain KCs and LSECs homeostasis by producing cholesterol and ketone bodies; HSCs participate in xenobiotics metabolism as an agent deliverer; KCs and LSECs mediate immune response through MHC class II-TLRs and MHC class I-TGFβ cascade, respectively; and KCs play a central role in diurnal rhythms regulation through sensing diurnal IGF and temperature flux. Together, this work expands our understandings of liver physiology and provides a useful resource for future analyses of normal and diseased livers.
Sample scope Monoisolate
Release date 2023-06-07
Publication
PubMed ID Article title Journal name DOI Year
A Cytological Atlas of the Human Liver Proteome from PROTEOMESKY-LIVERHu 2.0, a Publicly Available Database Journal of Proteome Research 10.1021/acs.jproteome.2c00190 2022
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) Key Program 2018YFA0507500
Submitter PUMIN ZHANG (pzhangbcm@zju.edu.cn)
Organization Beijing Proteome Research Center
Submission date 2023-06-04

Project Data

Resource name Description