| Accession |
PRJCA017530 |
| Title |
Integrating single-cell and spatial transcriptomics reveals endoplasmic reticulum stress-related CAF subpopulations associated with chordoma progression |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
Single cell sequencing
Spatial transcriptome
|
| Organisms |
Homo sapiens
|
| Description |
The present study depicted the cancer-associated fibroblasts (CAFs) landscape of chordoma using transcriptomic sequencing and QIF approaches. We identified a unique cluster of CAF in recurrent tumors that highly expressed hypoxic genes and was functionally enriched in endoplasmic reticulum stress (ERS). The spatial transcriptomics data confirmed the existence of ERS-CAFs subtype and revealed that this cluster displayed the highest probability of proximity to tumor cells. The bulk RNA-seq and multiplexed quantitative immunofluorescence data indicated the crucial roles of ERS-CAFs in the progression of chordoma. Moreover, the ERS-CAF subpopulation contributed to chordoma progression possibly via direct crosstalk with tumor cells. These data suggested the possibility of targeting ERS-CAF for the treatment of chordoma. |
| Sample scope |
Single cell |
| Release date |
2023-09-15 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 37772937
|
Integrating single-cell and spatial transcriptomics reveals endoplasmic reticulum stress-related CAF subpopulations associated with chordoma progression
|
Neuro-Oncology
|
10.1093/neuonc/noad173
|
2023
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China
|
Young Scientists Fund
|
82002364
|
|
| National Natural Science Foundation of China (NSFC)
|
Young Scientists Fund
|
82003802
|
|
| Natural Science Foundation of Hunan Province
|
General Program
|
2019JJ50542
|
|
| Natural Science Foundation of Hunan Province
|
General Program
|
2021JJ40509
|
|
| Hunan Provincial Natural Science Foundation
|
Excellent Youth Scholars
|
2023JJ20035
|
|
| Clinical Research 4310 Program of the First Affiliated Hospital of the University of South China
|
General Program
|
20224310NHYCG04
|
|
| Clinical Research of Hunan Provincial Health Commission
|
General Program
|
20201978
|
|
| Clinical Research of Hunan Provincial Health Commission
|
General Program
|
20201962
|
|
| Research Foundation of Education Bureau of Hunan Province
|
General Program
|
20201956
|
|
| China Scholarship Council
|
General Program
|
22B0441
|
|
|
|
| Submitter |
Mingxiang
Zou (zoumx_spine@usc.edu.cn)
|
| Organization |
First affiliated hospital of university of South China |
| Submission date |
2023-06-02 |
