1. Home
  2. Browse
  3. PRJCA017779
项目编号 PRJCA017779
项目标题 Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids
涉及领域 Medical
数据类型 Epigenomics
Single cell sequencing
物种名称 Homo sapiens
描述信息 Studies of cultured embryos have provided insights into human peri-implantation development. However, detailed knowledge of peri-implantation lineage developments as well as underlying mechanisms remain obscure. Using 3D-cultured human embryos, herein we report a complete cell atlas of the early post-implantation lineages and decipher cellular composition and gene signatures of the epiblast and hypoblast derivatives. In addition, we develop an embryo-like assembloid (E-assembloid) by assembling naive hESCs and extraembryonic cells. Using human embryos and E-assembloids, we reveal that WNT, BMP and Nodal signaling synergistically, but functionally differently, orchestrate human peri-implantation lineage development. Specially, we dissect mechanisms underlying extraembryonic mesoderm and extraembryonic endoderm specifications. Finally, an improved E-assembloid is developed to recapitulate the epiblast and hypoblast developments and tissue architectures in the pre-gastrulation human embryo. Our findings provide insights of the human peri-implantation development, and the E-assembloid offers a useful model to disentangle cellular behaviors and signaling interactions that drive human embryogenesis.
样品范围 Single cell
发布日期 2023-06-19
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2022YFA1103100
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2021YFA0805700
National Natural Science Foundation of China (NSFC) Key Program 32130034
National Natural Science Foundation of China (NSFC) Major Program 82192874
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2020YFA0112700
Yunnan Province Science and Technology Department Major Basic Research Project of Science and Technology of Yunnan 202102AA100007
Yunnan Province Science and Technology Department Major Basic Research Project of Science and Technology of Yunnan 202001BC070001
Yunnan Province Science and Technology Department Major Basic Research Project of Science and Technology of Yunnan 2019FY002
Kunming Medical University Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University 202101AY070001-260
Kunming Medical University Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University 202301AY070001-298
提交者 tianqing Li (litq@lpbr.cn)
提交单位 Institute of Primate Translational Medicine, Kunming University of Science and Technology
提交日期 2023-06-19

项目包含数据信息

资源名称 描述
BioSample (47)  show -