| Accession |
PRJCA017831 |
| Title |
Megakaryocytes regulate the development of hematopoietic stem cell precursors |
| Relevance |
Model organism |
| Data types |
Transcriptome or Gene expression
Single cell sequencing
|
| Organisms |
Mus musculus
|
| Description |
The study of the physiological function of megakaryocytes is one of the hotspots in the field of hematopoietic research. Previous studies have shown that megakaryocytes are involved in multiple biological processes, such as platelet production, coagulation,inflammation and maintenance of adult bone marrow hematopoietic stem cell homeostasis. In embryos, megakaryocytes are one of the earliest hematopoietic cells. It has been reported that megakaryocytes are involved in regulating the development of blood vessels in the embryonic head and placenta in the late-stage embryo. However, little is known about its regulatory role in embryonic hematopoietic stem cell development. Therefore, based on our familiar with regulatory characteristics of hematopoietic development at different sites (embryonic head and aorta-gonad-mesonephros), this project intends to use a megakaryocyte specific reporter mouse model to analyze the developmental dynamics of megakaryocytes at different hematopoietic sites and to describe the regulation of megakaryocytes on embryonic hematopoiesis and the cell types interacted with megakaryocytes by using the megakaryocyte ablation mouse model; the corresponding hematopoietic-related cell types and megakaryocytes will be sorted for single-cell omics analysis and the molecules that megakaryocytes regulate hematopoiesis will be discovered. Combined with the in vitro and in vivo research methods of hematopoietic stem cells, the targeted molecules of megakaryocytes in regulating hematopoietic development at different sites will be described and the new molecular mechanism of hematopoietic development regulation will be explained, which provides a new technological breakthrough for the in vitro regeneration of hematopoietic stem cells and promotes the development of regenerative medicine. |
| Sample scope |
Single cell |
| Release date |
2024-09-27 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 38580775
|
A subset of megakaryocytes regulates development of hematopoietic stem cell precursors
|
The EMBO Journal
|
10.1038/s44318-024-00079-4
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
82270119
|
|
| National Key Research and Development Program
|
|
2019YFA0801802
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Research and Development Program of China
|
2019YFA0801800
|
|
|
|
| Submitter |
Li
Zhuan (zhuanli2018@smu.edu.cn)
|
| Organization |
Southern Medical University |
| Submission date |
2023-02-08 |
