| 项目编号 | PRJCA017857 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 项目标题 | Single-cell multi-omics sequencing reveals the epigenomic landscapes in human spermatogenesis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 涉及领域 | Medical | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Raw sequence reads Single cell sequencing |
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| 物种名称 |
Mus musculus
Homo sapiens |
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| 描述信息 | Human spermatogenesis is a well-ordered dynamic process, which ensures the long-term production of functional spermatozoa; however, the roles of DNA methylation and chromatin accessibility in this process remain largely unknown. Here, by simultaneously investigating the chromatin accessibility, DNA methylome and transcriptome landscapes of human spermatogenesis using the modified single-cell chromatin overall omic-scale landscape sequencing approach (Modified scCOOL-seq), we revealed that the transcriptional changes throughout human spermatogenesis were correlated with the dynamic alternations of chromatin accessibility; particularly, several potential transcription factors and a set of regulatory elements involved in such process were identified. Remarkably, a round of DNA demethylation was uncovered upon meiosis initiation in human spermatogenesis, which was associated with male meiotic recombination and conserved in human and mouse. And aberrant DNA hypermethylation at recombination hotspots could be detected in leptotene spermatocytes of certain nonobstructive azoospermia patients. Functionally, the intervention of DNA demethylation affected male meiotic recombination. Collectively, this study provides multi-omics landscapes of human spermatogenesis at single-cell resolution, and offers insights into the association between DNA demethylation and male meiotic recombination. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 样品范围 | Multispecies | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 发布日期 | 2023-07-09 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Xiaoyang Zhao (zhaoxiaoyang@smu.edu.cn) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 提交单位 | Southern Medical University | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 提交日期 | 2023-06-22 |