| 项目编号 |
PRJCA018031 |
| 项目标题 |
Schwann Cells Regulate Tumor Cells and Cancer-Associated Fibroblasts in the Pancreatic Ductal Adenocarcinoma Microenvironment |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing
microarray-based spatial transcriptome
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Neuropathy is a feature more frequently observed in pancreatic ductal adenocarcinoma (PDAC) than other tumors. Schwann cells, the most prevalent cell type in peripheral nerves, migrate toward tumor cells and associate with poor prognosis in PDAC. To unveil the effects of Schwann cells on the neuro-stroma niche, here we perform single-cell RNA-sequencing and microarray-based spatial transcriptome analysis of PDAC tissues. Results suggest that Schwann cells may drive tumor cells and cancer-associated fibroblasts (CAFs) to more malignant subtypes: basal-like and inflammatory CAFs (iCAFs), respectively. Moreover, in vitro and in vivo assays demonstrate that Schwann cells enhance the proliferation and migration of PDAC cells via Midkine signaling and promote the switch of CAFs to iCAFs via interleukin-1a. Culture of tumor cells and CAFs with Schwann cells conditional medium accelerates PDAC progression. Thus, we reveal that Schwann cells induce malignant subtypes of tumor cells and CAFs in the PDAC milieu. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2023-06-30 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
81470894
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82073326
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81871906
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81802316
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82173226
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82173219
|
|
|
|
| 本项目在其他数据库系统中的项目序列号 |
| 项目序列号 |
数据库名称 |
| GSE202742
|
NCBI
|
| GSE202740
|
NCBI
|
| GSE201601
|
NCBI
|
|
|
| 提交者 |
Hao
Chen (haochendr@126.com)
|
| 提交单位 |
Ruijin Hospital, Shanghai jiaotong University School of Medicine |
| 提交日期 |
2023-06-30 |
