| 项目编号 | PRJCA018124 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 项目标题 | ADNP Modulates SINE B2-Derived CTCF-Binding Sites during Mouse Embryonic Blastocyst Formation | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 涉及领域 | Model organism | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 数据类型 |
Epigenomics
Transcriptome or Gene expression Raw sequence reads |
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| 物种名称 | Mus musculus | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 描述信息 | During early embryo development, the nuclear factor CTCF plays a vital role in organizing chromatin structure and regulating transcription. Previous studies have examined the establishment of nucleosome profiles around the CTCF motifs shortly after fertilization. However, the kinetics of chromatin occupation in pre-implantation embryos have remained unclear. In this study, we utilized CUT&RUN technology to investigate CTCF occupancy in mouse pre-implantation development. Our findings revealed that CTCF begins binding to the genome prior to zygotic genome activation (ZGA), with a preference for CTCF anchored chromatin loops. Although the majority of CTCF occupancy is consistently maintained, we identified a specific set of binding sites enriched in the mouse-specific short-interspersed element (SINE) family B2, which are restricted to the cleavage stages. Notably, we discovered that the neuroprotective protein ADNP counteracts the stable association of CTCF at SINE B2-derived CTCF-binding sites. Knockout of Adnp in the zygote led to impaired cell differentiation, erasure of H3K9me3 and transcriptional derepression of SINE B2 during the morula-to-blastocyst transition. Our analysis highlighted an ADNP-dependent restriction of CTCF-binding during cell differentiation in pre-implantation embryos. Furthermore, our study provided new insights into the regulation of transcription factor (TF) binding kinetics on exapted sites arising from transposable element (TE) expansions in early embryos. These findings shed light on the functional importance of TEs in promoting genetic innovation and actively shaping the early embryo developmental process specific to mammals. | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 发布日期 | 2025-02-06 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Shaorong Gao (gaoshaorong@tongji.edu.cn) | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 提交单位 | Tongji University | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 提交日期 | 2023-07-05 |