| 项目编号 | PRJCA018235 | ||||||||||||||||
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| 项目标题 | Allele-aware Chromosomal-Level Genome Assembly Reveals Regulatory Variations in Mulberry Fruit Anthocyanin Content | ||||||||||||||||
| 涉及领域 | Agricultural | ||||||||||||||||
| 数据类型 |
Whole genome sequencing
Transcriptome or Gene expression Genome sequencing and assembly Raw sequence reads Genome sequencing Assembly |
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| 物种名称 |
Morus alba var. atropurpurea
Morus alba Morus |
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| 描述信息 | Mulberry (genus Morus) is a plant with over 1,000 varieties, exhibiting a wide range of fruit colors and abundant anthocyanin content, which hold significant economic and nutritional value. Nevertheless, limited genetic diversity and high heterozygosity in modern mulberry cultivars restrict our understanding of fruit coloration. Here, we present haplotype-based chromosomal landscapes of two mulberry cultivars, Morus atropurpurea cv. 'Zhongsang5801' (ZS5801) and Morus alba cv. 'Zhenzhubai' (ZZB), with significant differences in anthocyanin content, using high-fidelity sequencing and chromatin conformation capture sequencing. The assembled genomes of mulberry spanned respectively 293.6-301.3 Mb and 309-314.9 Mb, with contig N50 values ranging from 2.6 Mb to 8.9 Mb across 14 chromosomes. Leveraging the differences between haplotypes, we identified a significantly allelic dominant chalcone synthase gene whose preference expression positively correlated with anthocyanin content during the development of black-purple fruits in ZS5801. Comparative genomic analysis revealed both expansion and convergence of the FLS and DFR enzyme gene families in the anthocyanin biosynthesis pathway between the two cultivars. Metabolomic profiling confirmed that variations in anthocyanin content primarily accounted for the observed color differences in fruits. Transcriptomic and co-expression analysis validated the metabolomic findings, shedding light on gene expression patterns during fruit development and identifying key genes involved in fruit coloration and regulatory changes. Furthermore, whole-genome resequencing and genome-wide association analysis of 112 mulberry samples led to the identification of a novel locus, the MaVHAG3 gene, which is responsible for anthocyanin accumulation and potentially adaptation to higher latitudes in mulberry fruits. In conclusion, this study employed a multi-omics approach to unravel the intricate regulation of mulberry fruit color, providing valuable genetic resources for investigating domestication mechanisms and genome-based functional genetics and molecular breeding of mulberry. | ||||||||||||||||
| 样品范围 | Multiisolate | ||||||||||||||||
| 发布日期 | 2023-10-31 | ||||||||||||||||
| 项目资金来源 |
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| 提交者 | Aichun Zhao (zhaoaichun@swu.edu.cn) | ||||||||||||||||
| 提交单位 | Southwest University | ||||||||||||||||
| 提交日期 | 2022-04-27 |