| Accession | PRJCA018236 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Title | A weak allele of enhancer GE1 selected during tomato domestication for low toxic glycoalkaloids | ||||||||
| Relevance | Agricultural | ||||||||
| Data types |
Whole genome sequencing
Raw sequence reads Hi-C |
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| Organisms | Solanum lycopersicum | ||||||||
| Description | The genetic based that guided negative selection of steroid glycoalkaloids (SGA) production during tomato domestication remain poorly understood. Here, we identified a distal enhancer, GAME Enhancer 1 (GE1) , as key regulator of SGA metabolism in tomato. GE1 recruits MYC2-GAME9 transcriptional complex to regulate the expression of GAME cluster genes via the formation of a chromatin loop located in the neighboring DNA region. A naturally occurring GE176 allelic variant proved more active in stimulating GAME genes' expression. We show that a weaker GE1 allele has been the main driver for selecting reduced SGA levels during tomato domestication. Unravelling the "TFs-Enhancer-Promoter" regulatory mechanism operating in SGAs metabolism opens unprecedented prospects for SGA manipulation in Solanaceae via precision breeding strategies. | ||||||||
| Sample scope | Multiisolate | ||||||||
| Release date | 2024-03-05 | ||||||||
| Grants |
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| Submitter | Peng Shu (sp1373751728@163.com) | ||||||||
| Organization | Sichuan University | ||||||||
| Submission date | 2023-07-10 |