| 项目编号 |
PRJCA018941 |
| 项目标题 |
GSTM2 alleviates heart failure via inhibiting DNA damage in cardiomyocytes |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Heart failure (HF) seriously threatens human's health world widely. However, the pathological mechanisms of HF are still not fully clear. In this study, we performed proteomics and transcriptomics analysis to overview the detailed changes of proteins and mRNAs in HF with human heart failure samples and healthy donors, and found there was a great difference in the protein change pattern between the atrium and ventricle of HF myocardium. It is interested that metabolism model changed in ventricular tissue of HF samples, and inflammation pathways were activated in atrial tissues. Through analysis of the differentially expressed genes in the heart failure samples, we found that several glutathione S-transferases (GSTs) family members decreased in all ventricular samples, especially Glutathione S-transferase M2-2 (GSTM2). Furthermore, overexpression of GSTM2 effectively relieved cardiac hypertrophy progression in transverse aortic constriction (TAC) surgery-induced HF mouse model. Moreover, we found that GSTM2 attenuated DNA damage and extrachromosomal Circular DNA (eccDNA) production in cardiomyocytes, thereby alleviating interferon-I stimulated macrophages inflammation in heart tissue. Our study establishes a proteomic and transcriptomic map of human HF tissues, highlights the functional importance of GSTM2 in HF progression, and provides a novel therapeutic target for HF. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-02-02 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
Young Scientists Fund
|
82200269
|
|
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
82271812
|
|
| National Natural Science Foundation of China (NSFC)
|
Young Scientists Fund
|
82000345
|
|
|
|
| 提交者 |
Hongfei
Xu (xuhongfei@zju.edu.cn)
|
| 提交单位 |
The First Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China |
| 提交日期 |
2023-08-10 |
