| 项目编号 |
PRJCA019468 |
| 项目标题 |
Bacillus cereus is a key microbial determinant of the incidence of intractable otitis media with effusion |
| 涉及领域 |
Medical |
| 数据类型 |
Metagenome
|
| 物种名称 |
human metagenome
|
| 描述信息 |
Currently, the mechanisms by which otitis media with effusion (OME) progresses to intractable OME is unclear. Since crosstalk between microbiome and host contributes to many diseases, we hypothesized that similar interactions could occur in the middle ear effusion (MEE) samples from patients with OME and influence intractable OME pathogenesis. This study aimed to evaluate the microbial profile of MEE samples and to determine whether there were microbial differences between the MEE microbiota of patients with intractable OME and those with rapidly cured OME. MEE samples obtained from 46 OME patients (20 patients in the long course group and 26 patients in the short course group) were analyzed using metagenomic sequencing, and then differential microbes associated with the different disease courses were identified. The difference was verified by further experimental validation, including fluorescence in situ hybridization (FISH) and quantitative polymerase chain reaction (qPCR). The alpha diversity indices and overall MEE microbial structure showed no significant difference between the long course and short course groups, but species such as Bacillus cereus, Nocardiopsis dassonvillei and Rothia aeria were significantly more prevalent in the MEE of long course OME patients. qPCR analyses and FISH also confirmed the difference in the abundance of Bacillus cereus between the two groups. Bacillus cereus may play a role in the persistence of OME infection and could be used as a biomarker to predict OME prognosis. Patients with Bacillus cereus in MEE can be considered suitable for early intervention, such as with ventilation tube insertion. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2023-09-18 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40319164
|
Bacillus cereus is a key microbial determinant of intractable otitis media with effusion
|
Communications Medicine
|
10.1038/s43856-025-00876-w
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National High Level Hospital Clinical Research Funding
|
National High Level Hospital Clinical Research Funding
|
2022-PUMCH-B-094
|
|
|
|
| 提交者 |
Yue
Fan (chromefan@126.com)
|
| 提交单位 |
Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College |
| 提交日期 |
2023-09-02 |
