| Accession |
PRJCA019658 |
| Title |
Spatial transcriptome of the mouse cloaca |
| Relevance |
Model organism |
| Data types |
Transcriptome or Gene expression
Raw sequence reads
|
| Organisms |
Mus musculus
|
| Description |
Evolutionary and functional significance of the mammalian urinary bladder remains undervalued and ill-defined. Here, to determine the molecular architecture of the mouse cloaca, we collected mouse E11.5 embryo and profiled the spatial transcriptome using Geo-seq. Spatial transcriptomics reveal that Wnt2 is expressed in the triangular cloacal mesenchyme domain flanked by the umbilical arteries. Wnt2 is downregulated in Shh mutants, and knockout of Wnt2 causes reduced bladder size and single umbilical artery phenotypes. These findings collectively suggest that the Shh-Wnt2 pathway orchestrates co-development of the bladder and umbilical arteries. |
| Sample scope |
Monoisolate |
| Release date |
2023-09-21 |
| Biomaterial provider |
Cedars-Sinai Medical Center, Prof Xue Li's lab |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Basic Research Program of China (973 Program)
|
2019YFA0801402
|
|
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
32130030
|
|
| Chinese Academy of Sciences (CAS)
|
Strategic Priority Research Program of Chinese Academy of Sciences (CAS)
|
XDA16020501
|
|
|
|
| Submitter |
Xianfa
Yang (yxianfa@gmail.com)
|
| Organization |
Guangzhou National Laboratory |
| Submission date |
2023-09-10 |
