Accession PRJCA019658
Title Spatial transcriptome of the mouse cloaca
Relevance Model organism
Data types Transcriptome or Gene expression
Raw sequence reads
Organisms Mus musculus
Description Evolutionary and functional significance of the mammalian urinary bladder remains undervalued and ill-defined. Here, to determine the molecular architecture of the mouse cloaca, we collected mouse E11.5 embryo and profiled the spatial transcriptome using Geo-seq. Spatial transcriptomics reveal that Wnt2 is expressed in the triangular cloacal mesenchyme domain flanked by the umbilical arteries. Wnt2 is downregulated in Shh mutants, and knockout of Wnt2 causes reduced bladder size and single umbilical artery phenotypes. These findings collectively suggest that the Shh-Wnt2 pathway orchestrates co-development of the bladder and umbilical arteries.
Sample scope Monoisolate
Release date 2023-09-21
Biomaterial provider Cedars-Sinai Medical Center, Prof Xue Li's lab
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Basic Research Program of China (973 Program) 2019YFA0801402
National Natural Science Foundation of China (NSFC) Key Program 32130030
Chinese Academy of Sciences (CAS) Strategic Priority Research Program of Chinese Academy of Sciences (CAS) XDA16020501
Submitter Xianfa    Yang  (
Organization Guangzhou National Laboratory
Submission date 2023-09-10

Project Data

Resource name Description
BioSample (90)  show -
GSA (2) -
CRA013517 Spatial transcriptomics of the cloaca-2
CRA012566 Geo-seq of the mouse cloaca