| 描述信息 |
Human gastrulation establishes the blueprint for three germ layers and the body axis in the early embryo. Concurrently, diverse extraembryonic cellular lineages are segregated to support proper gastrula development and pattern formation. Comprehensive knowledge on the molecular code as well as the key events of embryonic and extraembryonic development in vivo, is crucial for comprehending human development and advancing stem-cell-based technologies. However, research on this stage is extremely challenging, particularly in humans, due to the inaccessibility and infrequent availability of in vivo embryos, as well as ethical concerns regarding in vitro cultured embryos. Here, we obtained a first in vivo intact gastrulating human embryo embryos corresponding to Carnegie stage eight (CS7), and performed serial transverse sectioning for single-cell stereo-seq, resulting in a comprehensive spatial transcriptomic dataset. |
