| Description |
Understanding the impact of different fertilization schemes on Euryales Semen planting and exploring the supporting mechanism are crucial for achieving high yield and sustainable development of the Euryales Semen planting industry. In this study, a field plot experiment was conducted to evaluate the effects of four different fertilization patterns (The unfertilized group, while the other three groups have organic fertilizer to chemical fertilizer ratios of 3:7, 5:5, and 7:3, respectively) on Euryales Semen yield and quality, using morphological characteristics and metabolomic changes. Furthermore, the study revealed the internal mechanisms of how different fertilization modes affect the yield and quality of Euryales Semen by analyzing soil physicochemical properties, soil enzyme activity, and soil microbial structure. The results showed that the quality and yield were the best when the ratio of organic fertilizer to chemical fertilizer is 7:3 and the flavonoid and organic acid contents were higher than those in the other treatment groups. The Euryales Semen yield, soil available nitrogen, soil available phosphorus, soil total nitrogen, soil organic matter, and soil enzyme activity showed a similar trend. When the organic fertilizer was mixed with chemical fertilizer in a 7:3 ratio, it resulted in increased soil microbial diversity and higher abundance of Proteobacteria, while reducing the abundance of Firmicutes. This treatment outperformed the others. Redundancy analysis showed that Chloroflexi, Gammaproteobacteria and hycreales-incertaishedis were significantly enriched in the ratio of organic fertilizer to chemical fertilizer of 7:3. An in-depth variance partitioning analysis illustrated that the combined contributions of soil properties, enzyme activities, and their interactions collectively accounted for 90.80% of the variations in Euryales Semen yield and metabolome. In general, blending organic and chemical fertilizers at a 7:3 ratio can enhance soil fertility, boost Euryales Semen yield and quality, and alter the ecological conditions for bacteria and fungi. |
