| 项目编号 |
PRJCA021123 |
| 项目标题 |
Control of Fusarium Head Blight of wheat with Bacillus velezensis E2 and potential mechanisms of action |
| 涉及领域 |
Agricultural |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
|
| 物种名称 |
Fusarium asiaticum
|
| 描述信息 |
Wheat growth is easily inhibited by Fusarium head blight (FHB) infection, which poses a huge threat to wheat storage and food safety. The use of antagonistic bacteria is a promising method for controlling the FHB pathogen. In this study, a fungal strain was isolated from diseased wheat and identified as Fusarium asiaticum F1 (F. asiaticum F1). Bacillus velezensis E2 (B.velezensis E2) isolated from a previous investigation in our laboratory, showed a notable inhibitory effect on F. asiaticum F1 growth and deoxynivalenol (DON) formation in grains. Spore germination was significantly reduced when treated with B.velezensis E2, and they appeared to have vesicular structures when examined under the microscope. Observations by using scanning electron microscopy (SEM) showed that the hyphae of F. asiaticum F1 were shrunken and broken when treated with B. velezensis E2. The RNA-seq results of F1 hyphae treated with B. velezensis E2 showed that differentially expressed genes (DEGs), which were involved in multiple metabolic pathways such as toxin synthesis, autophagy process, and glycan synthesis, especially the genes associated with DON synthesis were significantly downregulated. In summary, this study provides new insights and antagonistic mechanisms for the biological control of FHB. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2023-11-08 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 38921376
|
Control of Fusarium Head Blight of Wheat with Bacillus velezensis E2 and Potential Mechanisms of Action
|
Journal of Fungi
|
10.3390/jof10060390
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Key R&D Program of China
|
|
2022ZD0115801
|
|
| State Key Laboratory of Crop Biology in Shandong Agriculture University
|
|
2020KF07
|
|
| Key R&D Program of Jiangsu Province
|
|
BE2021338
|
|
| Funds for Independent Innovation of Agricultural Science & Technology in Jiangsu Province
|
|
CX223114
|
|
|
|
| 提交者 |
Jianing
Ma (majianing7581@163.com)
|
| 提交单位 |
Jiangsu University |
| 提交日期 |
2023-10-29 |
