| 项目编号 |
PRJCA021237 |
| 项目标题 |
Fostering the Transition to Human Ground-State Pluripotency through Precise NFκB/RELA Circuitry Inhibition |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
The transition from human primed pluripotency to a naive state offers insight into embryo implantation. We developed a serum-free system, 6iLV, achieving a 90% conversion rate in 12 days. Multi-omics sequencing revealed chromatin accessibility changes, with increased openness at OCT and KLF binding motifs and decreased accessibility at ZIC, RFX, and NFκB/RELA motifs. MicroRNA profiling showed elevated miR-372/373 and miR-182/183 clusters and decreased miR-302 and miR-363/106 cluster expression. Functional experiments demonstrated that miR-372/373 overexpression promoted the transition, while NFκB/RELA overexpression inhibited it. Mechanistically, miR-372/373 suppressed the expression of NFκkB/RELA, facilitating the transition. Our findings uncover a miR-372/373-NFkB/RELA regulatory axis guiding human naive pluripotency reconstruction. This study provides insights into the epigenetic dynamics of pluripotency transitions with potential implications for regenerative medicine and developmental biology. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2023-11-14 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2018YFE0204800
|
|
| National Natural Science Foundation of China (NSFC)
|
Excellent Young Scientists Fund
|
32022019
|
|
|
|
| 提交者 |
Jing
Liu (liu_jing@gibh.ac.cn)
|
| 提交单位 |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| 提交日期 |
2023-11-13 |
