| Accession |
PRJCA021458 |
| Title |
Single-Cell Sequencing Revealed The Heterogeneity of ALI Model and Epithelial Cell Alternations After Exposure to Electronic Cigarette Vapour |
| Relevance |
Medical |
| Data types |
Single cell sequencing
|
| Organisms |
Homo sapiens
|
| Description |
Electronic cigarettes (e-cigarettes) were advertised as a healthier alternative to traditional cigarettes. However, the exact effects of e-cigarettes on the bronchial epithelium are poorly understood. Air-liquid interface (ALI) culture enable human primary bronchial epithelial cells to differentiate into bronchial epithelium (ALI-HBE) and provides an in vitro model that simulate the biological characteristics of normal bronchial epithelium. In the current study, ALI-HBEs were used to reveal previously unrecognized transcriptional heterogeneity within the human bronchial epithelium and cell type-specific effects upon acute expoure to e-cigarette vapour (e-vapour) containing distinc components (nicotine and/or flavoring) by single-cell sequencing. Specifically, acute e-vapour exposure containing nicotine affected gene expression related to secretory function and basal to secretory transformation. In addition, acute e-vapour exposure containing flavoring might contribute to the virus infection susceptibility and activate the epithelial-mesenchymal transition. Taken together, heterogeneity and transcriptional characteristic of human bronchial epithelium were mirrored by the in vitro ALI-HBE model. Single-cell sequencing data provided high-resolution insights into the e-vapour-induced remodeling of bronchial epithelium. The data may also help to discover potential SARS-CoV-2 entry factors and therapeautic targets for restoring normal function of bronchial epithelium upon e-cigarette used. |
| Sample scope |
Single cell |
| Release date |
2024-11-01 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
|
|
Single-Cell RNA Sequencing Reveals Heterogeneity of ALI Model and Epithelial Cell Alterations after Exposure to Electronic Cigarette Vapor
|
Research Square
|
10.21203/rs.3.rs-3708689/v1
|
2023
|
| 39397927
|
Single-cell RNA sequencing reveals heterogeneity of ALI model and epithelial cell alterations after exposure to electronic cigarette aerosol
|
Heliyon
|
10.1016/j.heliyon.2024.e38552
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| No funding support
|
|
|
|
|
|
| Submitter |
Xiaofan
Mao (xiaofanmao@126.com)
|
| Organization |
The First People's Hospital of Foshan |
| Submission date |
2023-11-21 |
