| 项目编号 |
PRJCA021543 |
| 项目标题 |
Liver Mechanosignaling as a Natural Anti-Hepatitis B Virus Mechanism |
| 涉及领域 |
Medical |
| 数据类型 |
Raw sequence reads
Single cell sequencing
|
| 物种名称 |
Mus musculus
Homo sapiens
|
| 描述信息 |
The mechanisms underlying the natural control of hepatitis B virus (HBV) infection have long been an intriguing question. Given the wide physiological range of liver stiffness and the growing attention to the role of mechanical microenvironment in homeostasis and diseases, we investigated how extracellular matrix stiffness impacts HBV replication. High matrix stiffness significantly inhibited HBV replication and activated YAP, a key molecule in mechanosignaling. YAP activation notably suppressed HBV transcription and antigen expression, as confirmed in mouse models with elevated liver stiffness or YAP transduction. Several YAP-induced genes exhibited strong anti-HBV effects. In liver tissue from HBV-infected immune-tolerant and immune-active individuals, single-cell analysis revealed a significant negative correlation between YAP signature activation and HBV transcript levels. These findings unveil a novel mechanism that involves the mechanical environment of hepatocytes and YAP to control and clear viral infection in the liver, providing new perspectives for HBV cure studies and antiviral development. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2023-11-25 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2022YFA1303600
|
|
|
|
| 提交者 |
Jieliang
Chen (jieliangchen@fudan.edu.cn)
|
| 提交单位 |
Fudan University |
| 提交日期 |
2023-11-25 |
