| Accession |
PRJCA021663 |
| Title |
Transcriptome analysis reveals cadmium exposure enhanced the isoquinoline alkaloid biosynthesis and disease resistance in Coptis chinensis |
| Relevance |
Environmental |
| Data types |
Raw sequence reads
|
| Organisms |
Coptis chinensis
|
| Description |
Coptis chinensis Franch is a member of the Ranunculaceae family with a long history of medicinal use. Excessive cadmium (Cd) accumulation in its rhizome always hinders its medicinal application. But the molecular mechanisms behind Cd accumulation in C. chinensis are still unclear. In this study, transcriptome sequencing was used to investigate the gene expression responding to Cd in the roots, stems or leaves of C. chinensis. And Cd bioconcentration factors, translocation factors and 4 isoquinoline alkaloid contents in C. chinensis were also analyzed. Transcriptome sequencing results showed that genes expression involved in phenylpropanoid biosynthesis, isoquinoline alkaloid biosynthesis and plant-pathogen interaction of the C. chinensis were strongly enhanced in response to Cd exposure. The measurement of berberine, coptisine, palmatine, and epiberberine demonstrated that Cd stimulates the 4 isoquinoline alkaloids accumulation in roots. WGCNA result indicated that WRKY8 (Cluster-55763.31419) and WRKY47 (Cluster-55763.21590) may be the regulation nodes of secondary metabolic synthesis and plant-pathogen interaction in C. chinensis triggered by Cd. This study may provide insights into the molecular mechanism of Cd accumulation in C. chinensis and highlight the ecological significance of the active enrichment of Cd in medicinal plants. |
| Sample scope |
Synthetic |
| Release date |
2024-04-10 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 38218103
|
Transcriptome analysis reveals cadmium exposure enhanced the isoquinoline alkaloid biosynthesis and disease resistance in Coptis chinensis
|
Ecotoxicology and Environmental Safety
|
10.1016/j.ecoenv.2024.115940
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
81773835
|
|
| Key Research and Development Plan Projects of Shaanxi Province
|
|
2020ZDLSF05-02
|
|
|
|
| Submitter |
Xin
Li (18790137359@163.com)
|
| Organization |
Zhejiang Sci-Tech University |
| Submission date |
2023-11-30 |
