| 项目编号 |
PRJCA022445 |
| 项目标题 |
Spatially Single-cell Protein Landscape Reveals the Distinct Tumor Microenvironment Architecture and Communications in Hepatocellular Carcinoma |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Tumor microenvironment (TME) heterogeneity in hepatocellular carcinoma (HCC) on a spatial single-cell resolution remains unclearly. Here, we conducted co-detection by indexing (CODEX) to profile the spatial heterogeneity of 401 HCC samples with 36 biomarkers and determined 4,347,731 cells, 72 cell phenotypes (CP), and 40 cellular neighborhoods (CN). The synchronous activations of proliferation/hypoxia signal were presented in different CPs in certain CNs locally. Two mutually exclusive distributions (ring- or mosaic-like) were presented in different tumor cell-dominant CNs, of which mosaic-like enriches metabolic-related molecules. Five spatial patterns with distinct local CN compositions stratify clinical outcomes. Integration analysis with several independent cohorts revealed a novel Macrophage Vimentin+ population could promote tumor progression and immune suppression via interacting with Tregs and triggering IL-1β expression. In light of immunotherapeutic data, our study provides novel insights into the influence of TME architecture on tumor evolution, reinforcing the necessity of spatial heterogeneity for developing personalized therapeutic strategies. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-06-30 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
U21A20376
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82273277
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81902412
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81988101
|
|
| National Natural Science Foundation of China (NSFC)
|
|
91859205
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82002921
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81830054
|
|
| National Natural Science Foundation of Shanghai
|
|
21XD1404600
|
|
| National Natural Science Foundation of Shanghai
|
|
17ZR143800
|
|
| National Natural Science Foundation of Shanghai
|
|
21JC1406600
|
|
| National Natural Science Foundation of Shanghai
|
|
22140901000
|
|
|
|
| 提交者 |
lei
chen (chenlein39@hotmail.com)
|
| 提交单位 |
Eastern hepatobiliary surgery hospital |
| 提交日期 |
2023-12-28 |
