| 项目编号 |
PRJCA022454 |
| 项目标题 |
The effect of MeCP2 overexpression on DNA methylation in mouse growing oocyte by WGBS |
| 涉及领域 |
Medical |
| 数据类型 |
Whole genome sequencing
Epigenomics
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
The DNA methylation is gradually acquired during oogenesis sustained by successful follicle development. However, the functional roles of methyl-CpG-binding protein 2 (MeCP2), an epigenetic regulator displaying specifical binding with methylated DNA, remains unknown in oogenesis. In this study, we found MeCP2 protein was highly expressed in primordial and primary follicle, but MeCP2 was almost undetectable in secondary follicles. However, in aged ovary, MeCP2 protein is significantly increased in both oocyte and granulosa cells. We performed whole genome bisulfite sequencing (WGBS) to analyze the effect of MeCP2 overexpression on DNA methylation in mouse growing oocyte. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-09-23 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 38578457
|
CRL4DCAF13 E3 ubiquitin ligase targets MeCP2 for degradation to prevent DNA hypermethylation and ensure normal transcription in growing oocytes
|
Cellular and Molecular Life Sciences
|
10.1007/s00018-024-05185-4
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
Young Scientists Fund
|
31701260
|
The mechanism of ubiquitin ligase substrate recognition protein DCAF13 regulating histone H3K9me3 modification level and participating in early embryonic lineage differentiation
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
82071640
|
Mechanism and intervention study of DCAF8 involvement in aging of advanced oocytes
|
|
|
| 提交者 |
Yinli
Zhang (zhangyinli@zju.edu.cn)
|
| 提交单位 |
Sir Run Run Shaw Hospital, Zhejiang University School of Medicine |
| 提交日期 |
2023-12-28 |
