| Accession | PRJCA022795 | ||||||||||
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| Title | NADase CD38 is a key determinant of ovarian aging | ||||||||||
| Relevance | Medical | ||||||||||
| Data types |
Transcriptome or Gene expression
Single cell sequencing |
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| Organisms | Mus musculus | ||||||||||
| Description | The ovary ages earlier than most other tissues in women, yet the underlying biological mechanisms and strategies to delay ovarian aging remain enigmatic. In this study, a comprehensive analysis of transcriptomic landscapes across different organs in young and middle-aged mice unveiled early expression of age-associated genes in ovaries. This analysis identified heightened NADase CD38 expression and reduced NAD+ levels in middle-aged mice ovaries. Bulk and single-cell RNA sequencing demonstrated that CD38 deletion counteracted ovarian aging, preserving fertility and maintaining follicle reserve in aged mice by addressing age-related gene expression changes and intercellular communication disruptions. In our research, we conducted a comparative transcriptomic analysis of various organs, including the heart, kidney, brain, lung, liver, and muscle, using bulk RNA-Seq from young and middle-aged mice at 2 and 8 months of age, respectively. Additionally, we performed single-cell RNA-Seq on ovaries from young (2 months), old (12 months) WT, and old CD38-/- mice. To delve deeper into the effects of CD38 on MII quality, we also collected oocyte transcriptomes from young (2 months) and old (12 months) WT and old CD38-/- mice using smart-Seq | ||||||||||
| Sample scope | Single cell | ||||||||||
| Release date | 2024-01-12 | ||||||||||
| Publication |
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| Grants |
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| Submitter | Yingpu Sun (syp2008@vip.sina.com) | ||||||||||
| Organization | The First Affiliated Hospital of Zhengzhou University | ||||||||||
| Submission date | 2024-01-12 |