| 项目编号 |
PRJCA023488 |
| 项目标题 |
RNA-seq for TNF-stimulated endothelial cells |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
IFNγ-induced immunoinhibitory feedback is a crucial mechanism for endothelial cells to perform immunoregulation of T cells. However, whether TNFα is involved in regulating this program is unclear. Mouse vascular endothelial cells sEND.1 was taken for invitro experiment. After several passages and exclusion of mycoplasma contamination, sEND.1 cells were divided into two groups, including three samples in each group. One was treated by IFNγ (20ng/ml) as the control group (IFNγ), and another one was administrated with a combination treatment of TNFα (10ng/ml) and IFNγ (20ng/ml) as the experimental group (TNFα-IFNγ). 24h later, RNA was extracted from samples and sent to BGI for subsequent deep sequencing. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2024-02-06 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
YQRC2023005
|
|
|
|
| 提交者 |
Chen
Ni (nichen904@163.com)
|
| 提交单位 |
The First Affiliated Hospital of Zhengzhou University |
| 提交日期 |
2024-02-06 |
