| Description |
Floccularia luteovirens is a rare wild edible and medicinal fungus unique to the Qinghai-Tibet Plateau.Metabolomic analysis was performed using liquid chromatography-mass spectrometry (LC-MS) following established protocols. Quality control samples were prepared by pooling equal volumes of each sample to assess the stability and reproducibility of the untargeted metabolic analysis.The current untargeted metabolic analysis was performed LC-MS. For LC-MS, an ACQUITY UPLC I-Class plus (Waters, Shanghai, China) coupled with a QE plus (Thermo Fisher Scientific, Shanghai, China) high-resolution tandem mass spectrometer was used for LC-MS analysis. Separation was performed on a Waters ACQUITY UPLC HSS T3 .All raw data were imported into MS-DIAL software and Progenesis QI v3.0 software (Nonlinear, Dynamics, Newcastle, UK) for further identification processing. The missing values in the raw data were filled by using half of the minimum value. The detection of compounds by LC-MS was performed based on the Human Metabolome Database (HMDB), Metlin, PMDB, and self-built databases. |
