| Accession |
PRJCA023593 |
| Title |
EGCG intervention was administered in a murine model of colitis |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
16s
|
| Organisms |
Mus musculus
|
| Description |
Male C57BL/6J mice (8 weeks old) were orally gavaged with EGCG (80 mg/kg body weight) for either 21 or 24 days, while the remaining mice received an equivalent volume of physiological saline (200 uL). The dosage of EGCG was chosen based on the upper limit of exposure levels (300-866 mg/day) observed in high-dose consumers of green tea extracts in the adult population of the European Union. Animal equivalent doses were calculated by multiplying human doses (mg/kg) by the Km ratio (Km = 12.3). Colitis was induced by administering a 2.5% (w/v) solution of DSS (MP Biomedicals) in drinking water for 5 days, followed by 2 days of normal water consumption. At the end of the experiment, mice were euthanized under general anesthesia by cervical dislocation.Careful dissection of the colon and collection of colonic feces using aseptic freezing method for subsequent 16S sequencing. Colon tissues were carefully dissected, and 1 cm segments were immediately flash frozen in liquid nitrogen and dry ice for subsequent RNA extraction and sequencing at a sequencing facility. |
| Sample scope |
Monoisolate |
| Release date |
2024-09-12 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 39116526
|
EGCG drives gut microbial remodeling-induced epithelial GPR43 activation to lessen Th1 polarization in colitis
|
Redox Biology
|
10.1016/j.redox.2024.103291
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2023YFF1001900
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32002188
|
|
|
|
| Submitter |
beibei
Qin (3235468455@qq.com)
|
| Organization |
Hunan Agricultural University |
| Submission date |
2024-02-15 |
