| Description |
Bone morphogenetic protein 2 (BMP2) has been reported to regulate adipogenesis, but its role in porcine beige adipocyte formation remains unclear. Our data revealed that BMP2 was significantly induced at the early stages of porcine beige adipocyte differentiation. Additionally, supplementing rhBMP2 during the early stages, but not late stages of differentiation significantly enhanced porcine SVFs adipogenesis, thermogenesis, and proliferation. Furthermore, compared to the empty plasmid transfected-SVFs, overexpressed-BMP2 SVFs enhance lipid accumulation and thermogenesis, while knockdown-BMP2 SVFs have the opposite effect. RNA-seq of the above three types of cells revealed the enrichment of annotation of thermogenesis, brown cell differentiation et al. Besides, the analysis also highlighted the significant enrichment of cell adhesion, the MAPK cascade, and PPARγ signaling. Mechanistically, BMP2 positively regulates the adipogenic and thermogenic capacities of porcine beige adipocytes by activating PPARγ expression through AKT/mTOR and MAPK signaling pathways. |
