| Accession | PRJCA024217 | ||||||||||||||||
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| Title | Whole-genome sequencing, PacBio long-read sequencing and single-cell RNA-seq of precancerous colorectal lesions in mouse models | ||||||||||||||||
| Relevance | Evolution | ||||||||||||||||
| Data types |
Whole genome sequencing
Genome sequencing Single cell sequencing PacBio Sequel IIe |
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| Organisms | Mus musculus | ||||||||||||||||
| Description | To elucidate the somatic lineages and evolutionary dynamics in preneoplastic lesions, we established a transgenic mouse line carrying the lineage tracing barcode and used it to generate lineage traced neoplastic lesions using two mouse models (an azoxymethane (AOM) and dextran sodium sulfate (DSS) mouse model and an ApcMin/+ mouse model) of colorectal tumorigenesis. Through integrated analyses of single cell phylogenies (n=105 samples), whole-genome sequencing (n=31 mouse samples) and single-cell RNA sequencing (n=9 mouse samples), we have investigated the trajectory of intestinal tumorigenesis observed in both mouse models. | ||||||||||||||||
| Sample scope | Monoisolate | ||||||||||||||||
| Release date | 2024-07-31 | ||||||||||||||||
| Biomaterial provider | Zheng Hu | ||||||||||||||||
| Grants |
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| Submitter | Zheng Hu (zheng.hu@siat.ac.cn) | ||||||||||||||||
| Organization | Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences | ||||||||||||||||
| Submission date | 2024-03-08 |