| Description |
The UPLC-ESI-MS/MS analytical method was used for the qualitative and quantitative detection of the target metabolites, and the specific analytical conditions and analytical methods were as follows: The chromatographic column was ACQUITY UPLC BRHC18 (100*2.1mm, 1.7um), Waters; the high performance liquid chromatograph was Nexera UHPLC LC-30A, Shimadzu, Japan; Chromatographic conditions: Injection volume: 5 μL; Flow rate: 0.35 mL/min; Gradient Elution Procedures (GEP): 0 min A/B (90:10, V/V), 1 min A/B (90:10, V/V), 2 min A/B (75:25, V/V), 6 min A/B (65:35, V/V), 6.5 min A/B (5:95, V/V). 7.8 min A/B (5:95, V/V), 7.81 min A/B (90:10, V/V), 8.5 min A/B (90:10, V/V). A highly sensitive mass spectrometer AB Sciex Qtrap 5500, AB Sciex was used.Mass spectrometry conditions:Curtain gas: 35 (psi);Collision-activated dissociation (CAD) parameters: medium; Positive ion spray voltage: 5500V; negative ion spray voltage: -4500V;Ion source temperature: 450°C;Column temperature: 40°C;Spray gas (Gas1): 50(psi); auxiliary heating gas (Gas2): 60(psi). |
