| Accession | PRJCA024572 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Title | SETD1A/B regulates transcription-dependent H3K4me3 establishment and lineage segregation in mouse preimplantation embryos | ||||||||
| Relevance | Model organism | ||||||||
| Data types |
Epigenomics
Transcriptome or Gene expression |
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| Organisms | Mus musculus | ||||||||
| Description | To elucidate the specific histone methyltransferase accountable for H3K4me3 deposition in the early stages of mouse embryogenesis, we conducted H3K4me3 ChIP-seq analysis on Late2C and morula stage embryos following targeted knockdown of Mll2 and Setd1a/b. To examine the transcriptional impact on H3K4me3 dynamics, we performed Pol II and H3K4me3 ChIP-seq following a brief exposure to triptolide at intervals of 0, 2, 4, and 6 hours on embryos at the Late2C and morula stages, respectively. Furthermore, to explore the functional role of H3K4me3 during early embryonic development, we executed H3K4me3 ChIP-seq and RNA-seq analyses subsequent to either the knockdown or overexpression of enzymes responsible for the addition and removal of H3K4me3 marks, respectively. | ||||||||
| Sample scope | Monoisolate | ||||||||
| Release date | 2024-12-22 | ||||||||
| Grants |
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| Submitter | Jingjing Zhang (1263666013@qq.com) | ||||||||
| Organization | Huazhong Agricultural University | ||||||||
| Submission date | 2024-03-22 |
| Resource name | Description |
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| BioSample (87) show | - |