| Accession |
PRJCA024976 |
| Title |
scRNA-seq characterizing the heterogeneity of fibroblasts in breast cancer reveals a novel subtype SFRP4+ CAF that inhibits migration and predicts prognosis |
| Relevance |
Medical |
| Data types |
Single cell sequencing
|
| Organisms |
Homo
|
| Description |
Cancer-associated fibroblasts (CAFs) are a heterogeneous population of cells that influence the tumor microenvironment and the response to therapy in breast cancer (BC). Eight CAF subtypes were identified from single-cell RNA sequencing (scRNA-seq) in five tumor and six adjacent normal tissues. One of these subtypes, SFRP4+ CAFs, was novel and more prevalent in normal tissues. The existence of SFRP4+ CAFs were confirmed in two independent scRNA-seq datasets and a spatial transcriptomics dataset. We found that SFRP4+ CAFs inhibited BC cell migration and epithelial-mesenchymal transition (EMT) by secreting SFRP4 and modulating the WNT pathway. We also demonstrated that higher expression of SFRP4+ CAF markers was associated with better survival rate in BC while worse neoadjuvant chemotherapy treatment response in triple-negative breast cancer patientsand treatment response in BC patients. Our study characterized the heterogeneity of CAFs in BC and showed a new SFRP4+ CAF subtype that suppresses BC migration, providing a potential biomarker for precision prognosis and therapy. |
| Sample scope |
Single cell |
| Release date |
2024-04-05 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 38686196
|
scRNA-seq characterizing the heterogeneity of fibroblasts in breast cancer reveals a novel subtype SFRP4+ CAF that inhibits migration and predicts prognosis
|
Frontiers in Oncology
|
10.3389/fonc.2024.1348299
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
82172356
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81972003
|
|
|
|
| Submitter |
Ning
Lvwen (luwening@gmail.com)
|
| Organization |
The University of Hong Kong |
| Submission date |
2024-04-05 |
