| 项目编号 | PRJCA025083 | ||||||||
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| 项目标题 | Microbiota regulates the TET1-mediated DNA hydroxymethylation program in innate lymphoid cell differentiation | ||||||||
| 涉及领域 | Model organism | ||||||||
| 数据类型 | Genome sequencing | ||||||||
| 物种名称 | mouse gut metagenome | ||||||||
| 描述信息 | DNA was extracted from faeces of Zbtb16-Cre (Ctrl group) and Tet1flox/flox;Zbtb16-Cre mice with QIAamp DNA Mini Kit. Sample collection and preservation buffer served as negative control. Ribosomal 16S rDNA V4 region was amplified with universal primers: 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR reactions were performed in three replicates and purified with GeneJET TM Gel Extraction Kit. Sequencing libraries were generated with Ion Plus Fragment Library Kit. The libraries were sequenced on IonS5TM XL platform for generating 400-bp single-end reads. | ||||||||
| 样品范围 | Environment | ||||||||
| 发布日期 | 2024-04-10 | ||||||||
| 项目资金来源 |
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| 提交者 | zhang xu sheng (xushengzhang93@163.com) | ||||||||
| 提交单位 | Institute of Microbiology, Chinese Academy of Sciences | ||||||||
| 提交日期 | 2024-04-08 |