| Accession | PRJCA025083 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Title | Microbiota regulates the TET1-mediated DNA hydroxymethylation program in innate lymphoid cell differentiation | ||||||||
| Relevance | Model organism | ||||||||
| Data types | Genome sequencing | ||||||||
| Organisms | mouse gut metagenome | ||||||||
| Description | DNA was extracted from faeces of Zbtb16-Cre (Ctrl group) and Tet1flox/flox;Zbtb16-Cre mice with QIAamp DNA Mini Kit. Sample collection and preservation buffer served as negative control. Ribosomal 16S rDNA V4 region was amplified with universal primers: 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR reactions were performed in three replicates and purified with GeneJET TM Gel Extraction Kit. Sequencing libraries were generated with Ion Plus Fragment Library Kit. The libraries were sequenced on IonS5TM XL platform for generating 400-bp single-end reads. | ||||||||
| Sample scope | Environment | ||||||||
| Release date | 2024-04-10 | ||||||||
| Grants |
|
||||||||
| Submitter | zhang xu sheng (xushengzhang93@163.com) | ||||||||
| Organization | Institute of Microbiology, Chinese Academy of Sciences | ||||||||
| Submission date | 2024-04-08 |