| 项目编号 |
PRJCA025174 |
| 项目标题 |
Spatially resolved deep isotope tracing reveals inter-tissue metabolic crosstalk |
| 涉及领域 |
Medical |
| 数据类型 |
Metabolomics
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Organs collaborate to maintain metabolic homeostasis in mammals. Spatial metabolomics makes strides in profiling the metabolic landscape, yet can not directly inspect the metabolic crosstalk between tissues. Here, we introduce an approach to comprehensively trace the metabolic fate of 13 C-nutrients within the body and present a robust computational tool, MSIFluxer, to deep-probe metabolic activity in a spatial manner. Using ambient mass spectrometry imaging and isotope tracing, MSIFluxer empowers us to depict organ-specific metabolic characteristics by discerning the spatial distribution differences between newly synthesized labeled metabolites and native unlabeled ones. Importantly, we reveal that fatty acid metabolic crosstalk occurs between the liver and heart. Furthermore, we disclose metabolic coordination is lost system-wide in tumor-bearing mice and the host hexosamine biosynthesis pathway is considerably impacted by tumor burden. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-04-10 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
21927808
|
|
|
|
| 提交者 |
Xinzhu
Li (stxinzhu@outlook.com)
|
| 提交单位 |
Institue of Materia Medica Chinese Academy of Medical Science |
| 提交日期 |
2024-04-10 |
