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项目编号 PRJCA025291
项目标题 Time-resolved Single-Cell Transcriptomic Analysis of Wheat Responses to Adapted and Non-adapted Fusarium Infection
涉及领域 Agricultural
数据类型 Transcriptome or Gene expression
Raw sequence reads
Single cell sequencing
物种名称 Triticum aestivum
描述信息 Phytopathogenic fungi can be recognized by the plant immune system and trigger host defenses, but only adapted pathogens cause susceptibility. How different cell types orchestrate biological processes in response to heterogeneous colonization of organs by adapted and non-adapted pathogens remains largely unknown. Here we employed single-cell RNA sequencing to dissect the response of wheat coleoptiles to infection by the adapted fungal pathogen Fusarium graminearum (Fgr) and the non-adapted fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) at 1-, 2-, and 3-days post inoculation. We profiled the transcriptomes of over 90,000 cells and identified eight major cell types of the coleoptile: stomata, epidermis, chlorenchyma, parenchyma, outer sheath, inner sheath, phloem, and procambium. Differential expression analyses showed that the capacity of different cell types to respond to fungal infection varied. The upregulation of immune pathways was compartmentalized in nonhost resistance to Foc, but widespread in susceptible interaction with Fgr. Pseudotime analyses revealed continuous cell state transitions in the disease progression of infected cell types. Our work shows that the phloem and outer sheath are specialized cell types that collaborate for the rapid onset of nonhost resistance. However, Fgr induces a state of low transcriptional activity in chlorenchyma cells. Additionally, the competition between immune and susceptible processes in parenchyma results in specific cell states that are favored by the adapted pathogen Fgr. Overall, this work explains how cell types collaborate and are manipulated during fungal infections, providing insight into the intercellular mechanisms of plant immunity.
样品范围 Single cell
发布日期 2025-05-06
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
40845857 Single-cell transcriptomic analysis highlights specific cell types manipulated by Fusarium head blight fungus leading to wheat susceptibility Developmental Cell 10.1016/j.devcel.2025.07.022 2025
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Chinese Academy of Sciences (CAS) Strategic Priority Research Program of Chinese Academy of Sciences (CAS) XDB27040000
提交者 Dong Zhang (dzhang@cemps.ac.cn)
提交单位 CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology
提交日期 2024-04-10

项目包含数据信息

资源名称 描述
BioSample (35)  show -
GSA (2) -
CRA015977 Single-cell RNA sequencing data of wheat coleoptiles generated using BD Rhapsody system
CRA017730 RNA sequencing data of wheat coleoptiles using laser capture microdissection