| 描述信息 |
The classical color plant Loropetalum chinense var.rubrum was used as a model plant. In view of the industrial and scientific problems that the synthesis of anthocyanin was blocked, the accumulation in vacuoles was reduced and degraded, the leaf color became lighter, and the ornamental value and economic value became lower due to the unsuitable light environment, the LcHY51 gene was screened and cloned from the phenotypic, physiological and comparative transcriptomic analysis of Loropetalum chinense var.rubrum treated with different light quality. The results showed that the LcHY51 gene was transiently overexpressed. It significantly promoted the accumulation of anthocyanin in leaves of L.chinense.On this basis, we analyzed the temporal and spatial expression pattern of LcHY51 gene under blue light treatment, and explored its action site by combining its transcriptional activation activity and subcellular localization technology. The gene overexpression mutant and gene silencing mutant were created by forward genetics and reverse genetics. Transcriptome sequencing was performed under blue light induction, and the downstream genes interacting with LcHY51 gene were screened by comparative transcriptomics. Finally, the target genes interacting with LcHY51 were verified by yeast one-hybrid technology, yeast two-hybrid technology and double luciferase complementation technology, and the molecular mechanism of LcHY51 gene regulating the synthesis and metabolism of anthocyanin in Loropetalum chinense var.rubrum was revealed.Through the implementation of this project, in order to explore the response of Loropetalum chinense var.rubrum; provide theoretical and practical basis for the physiological and molecular mechanisms of light environment changes ; at the same time, it will be in Loropetalum chinense var.rubrum, Rhododendron, |
