项目编号 PRJCA025542
项目标题 Molecular mechanisms by which ccr3 regulates seed germination and dormancy in maize
涉及领域 Agricultural
数据类型 Transcriptome or Gene expression
Raw sequence reads
物种名称
描述信息 Pre-harvest germination (PHS) is a serious threat to the yield and quality of global crop seed production due to the lack of seed dormancy. PHS and seed dormancy as complex quantitative traits. Current genetic studies or breeding of crops with suitable seed dormancy are limited by fewer effectively regulated genes. Through the isolation and cloning of maize vp16 mutation sites and candidate genes, we confirmed that the candidate gene encodes Zmccr3, which is involved in lignin metabolism and regulates seed germination and dormancy. However, the molecular mechanism of its regulation is still unknown. Therefore, this project aimed to confirm the weighted co-expression network (WGCNA) regulated by Zmccr3 and clarify the metabolic network of Zmccr3 regulating grain germination and dormanness by using transcriptome analysis at two developmental stages of grain with differential vp16 locus (BC4F2). Transcriptomics, proteomics, physiological and biochemical tests were used to verify the metabolic pathways regulating grain germination and dormancy at different stages of grain development with differential vp16 loci. The completion of the project will elucidate the molecular mechanism of ZmcCR3-mediated lignin metabolism in regulating seed germination and dormancy. It can provide new regulatory pathways and new genes for maize seed germination and dormancy, and provide gene resources for molecular breeding of PHS, seed germination and dormancy.
样品范围 Multiisolate
发布日期 2026-01-30
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
Map-based cloning of Zmccr3 and its network construction and validation for regulating maize seed germination Research Square 10.21203/rs.3.rs-4841969/v1 2024
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Natural Science Foundation of Heibei Province of China General Program C2021204078
Hebei Provincial Department of Science and Technology General Program 21326328D
State Key Laboratory of North China Crop Improvement and Regulation General Program 23567601H
提交者 Liqing Feng (flq924311992@163.com)
提交单位 Hebei Agricultural University
提交日期 2024-04-23

项目包含数据信息

资源名称 描述
BioSample (48)  show -
GSA (1) -
CRA016997 Transcriptome data of Zmccr3