| Accession |
PRJCA026132 |
| Title |
The Effect of Microbial Inoculant on Microbial Community Diversity and Function in Rhizospheric Soil Under Tobacco Monoculture |
| Relevance |
Agricultural |
| Data types |
metabolomics data
|
| Organisms |
Nicotiana tabacum
|
| Description |
Over-application of chemical fertilizers and continuous cropping obstacles seriously restrict the sustainable development of tobacco production. Localized fertilization of beneficial microbes has potential advantages in achieving higher productivity, but the underlying biological mechanisms of interactions between rhizospheric microorganisms and the related metabolic cycle remain poorly characterized. Here, an integrative analysis of microbiomes with non-targeted metabolomics was performed on 30 soil samples of rhizosphere, root surrounding, and bulk soils from flue cured tobacco under continuous and non-continuous monocropping systems. The analysis was conducted using UPLC-MS/MS platforms and high-throughput sequencing targeting the bacterial 16S rRNA gene and fungal ITS gene. The microbial inoculant improved the cured leaf yield and disease resistance of tobacco and enhanced nicotine and nitrogen contents of tobacco leaves. The bacterial taxa Rhizobium, Pseudomonas, Sphingomonadaceae and Burkholderiaceae of the phylum Proteobacteria accumulated in high relative abundance and were identified as biomarkers following application of the microbial inoculant. Under continuous monocropping, metabolomics demonstrated that application of the microbial inoculant significantly affected the soil metabolite spectrum, and the differential metabolites were significantly enriched to the synthesis and degradation of nicotine (nicotinate and nicotinamide metabolism and biosynthesis of alkaloids derived from nicotinic acid). Additionally, microbes were closely related to the accumulation of metabolites through correlation analysis. The interactions between plant roots and rhizospheric microorganisms provide valuable information for understanding how these beneficial microbes affect complex biological processes and the adaption capacity of plants to environments. |
| Sample scope |
Multiisolate |
| Release date |
2024-06-28 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 38989997
|
Integrative analysis of microbiome and metabolome revealed the effect of microbial inoculant on microbial community diversity and function in rhizospheric soil under tobacco monoculture
|
Microbiology Spectrum
|
10.1128/spectrum.04046-23
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Applied Basic Research Project of China Tobacco Sichuan Industrial Co. Ltd
|
|
yl2020001
|
|
| Applied Basic Research Project of China Tobacco Sichuan Industrial Co. Ltd
|
|
SJ003-03
|
|
|
|
| Submitter |
Xianjun
Lai (laixianj@hotmail.com)
|
| Organization |
Xichang University |
| Submission date |
2024-05-13 |
