e.g., PRJCA000229; PRJNA784038; human
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Accession PRJCA026206
Title Highly specific and efficient RNA A-to-I editing
Relevance RNA base editing
Data types Raw sequence reads
Genome sequencing
Organisms Mus musculus
Homo sapiens
Description RNA editing can be a promising therapeutic approach. However, ectopic expression of RNA editing enzymes was found to trigger off-target editing, and the recruitment of endogenous adenosine deaminase acting on RNA (ADAR) suffers from low efficiency and fluctuating ADAR expression. Here, we identified ADAR inhibitors (ADIs) that suppressed the activity of the fused ADAR2 deaminase domain (ADAR2DD). Using ADI, we developed an RNA transformer adenine base editor (RtABE) with both high specificity and high efficiency. With the fusion of ADI to ADAR2DD, RtABE remains inactive until it binds its target site. After binding to the target site, ADI is cleaved from ADAR2DD and RtABE becomes active. RtABE induced efficient on-target editing in various cells with different ADAR expression levels. RtABE is a highly specific and efficient RNA editing system with broad applicability.
Sample scope Monoisolate
Release date 2025-02-07
Grants
Agency program Grant ID Grant title
National Key Research and Development Program National Key R&D Program of China 2023YFC3403402
National Key Research and Development Program National Key R&D Program of China 2018YFA0801401
National Key Research and Development Program National Key R&D Program of China 2019YFA0802804
National Natural Science Foundation of China (NSFC) 32371514
National Natural Science Foundation of China (NSFC) 31925011
National Natural Science Foundation of China (NSFC) 32070170
Ministry of Agriculture and Rural Affairs of the People's Republic of China NK2022010207
Shanghai Municipal Science and Technology Commission 21JC1404600
Shanghai Municipal Science and Technology Commission 23JS1400300
Shanghai Municipal Science and Technology Commission 23DX1900102
Shanghai Municipal Science and Technology Commission 23ZR1442500
Program of Shanghai Academic/Technology Research Leader 23XD1422500
Submitter Li YANG (liyang_fudan@fudan.edu.cn)
Organization Fudan University
Submission date 2024-05-16

Project Data

Resource name Description
BioSample (69)  show -
GSA (1) -
CRA016552 Whole-transcriptome sequencing for mouse liver tissue
GSA-Human (1)-
HRA007429Whole-transcriptome sequencing for 293FT cells