| 项目编号 |
PRJCA026452 |
| 项目标题 |
The mass spectrometry analysis of PARP1-associated proteins |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
After 48 hours of transfection, cells overexpressing Myc-PARP1 were harvested. Cells were lysed and immunoprecipitated with anti-Myc antibodies according to co-immunoprecipitation protocol. Then the immunoprecipitated proteins were subjected to Western blot. The bands corresponding to PARP1 were excised in gel digested with trypsin and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. MS experiments were performed on a nanoscale UHPLC system (EASY-Nlc1000 from Proxeon Biosystems, Odense, Denmark) connected to an Orbitrap Q-Exactive equipped with a nanoelectros pray source (Thermo Fisher Scientific, Bremen, Germany). The raw data were processed using Proteome Discoverer (PD, version 2.1), and MS/MS spectra were searched against the reviewed Swiss-Prot human proteome database. Only peptides with at least six amino acids in length were considered. The peptide and protein identifications were filtered by PD to control the false discovery rate (FDR) < 1%. At least one unique peptide was required for protein identification. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2024-05-25 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40316740
|
The deubiquitination-PARylation positive feedback loop of the USP10-PARP1 axis promotes DNA damage repair and affects therapeutic efficacy of PARP1 inhibitor
|
Oncogene
|
10.1038/s41388-025-03428-7
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82102740
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82073089
|
|
|
|
| 提交者 |
Hao
Li (haoli@cmu.edu.cn)
|
| 提交单位 |
China Medical University |
| 提交日期 |
2024-05-24 |
