| 项目编号 | PRJCA026810 | |||||||||||||||||||||||||||||||||||
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| 项目标题 | Dynamics of chromatin and transcriptome in the HSPCs at different hematopoietic developmental stages | |||||||||||||||||||||||||||||||||||
| 涉及领域 | Medicine and Biology | |||||||||||||||||||||||||||||||||||
| 数据类型 |
Whole genome sequencing
Epigenomics Transcriptome or Gene expression |
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| 物种名称 | Mus musculus | |||||||||||||||||||||||||||||||||||
| 描述信息 | ATAC-seq and RNA-seq were used to compare the chromatin states and corresponding transcriptomes of developing HSPC. We found that the chromatin states and transcriptomes change markedly when HSPCs migrate from PL to FL and from FL to BM. Then we compared chromatin among E12.5 PL, E12.5 FL, and E16.5 FL, the proportion of fragments in the nucleosome-free region significantly increases in E12.5 and E16.5 FL-HSPCs compared with E12.5 PL-HSPCs; the motifs of ETS and Runt are enriched at the opeing or closing peaks from E12.5 PL to E12.5 FL and E12.5 FL to E16.5 FL, and the expression of these factors is either up-regulated or down-regulated; the motifs of bZIP and Zf are enriched at the closing and opening peaks from E12.5 PL to E12.5 FL and E12.5 FL to E16.5 FL respectively. In line, the expression of these factors is down-regulated or up-regulated. | |||||||||||||||||||||||||||||||||||
| 样品范围 | Monoisolate | |||||||||||||||||||||||||||||||||||
| 发布日期 | 2024-09-05 | |||||||||||||||||||||||||||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Dengli Hong (dlhong@sjtu.edu.cn) | |||||||||||||||||||||||||||||||||||
| 提交单位 | Shanghai Jiaotong University School of Medicine | |||||||||||||||||||||||||||||||||||
| 提交日期 | 2024-06-05 |