| 项目编号 |
PRJCA026824 |
| 项目标题 |
DNA methylomic sequencing of Nautilus pompilius reveals an ancient and conserved epigenetic regulation pattern in cephalopoda |
| 涉及领域 |
DNA methylation |
| 数据类型 |
Epigenomics
Raw sequence reads
|
| 物种名称 |
Nautilus pompilius
|
| 描述信息 |
DNA methylation is a crucial epigenetic modification that plays a pivotal role in various biological processes. Although DNA methylation has been extensively studied for many years, most research has focused on traditional model organisms. Research on other species, especially invertebrates, remains relatively scarce. This study aimed to create a DNA methylation map for Nautilus pompilius, an ancient marine mollusk. First, homologous searches against N. pompilius genome identified key methyltransferases involved in DNA methylation: Dnmt1, Dnmt2 and Dnmt3. Then, whole-genome bisulfite sequencing (WGBS-seq) was used to gather information on DNA methylation in nautilus. Genome-wide methylation levels in nautilus are close to other invertebrates and a typical gene body methylation (gbM) was observed in nautilus. Characteristics of DNA methylation are largely conserved in nautilus compared to other invertebrates. The low methylation bias of the promoter and the first exon were also observed in nautilus. Overall, promoter methylation and gene body methylation may simultaneously affect gene expression in nautilus. Our research may contribute important information to the methylation map of invertebrates. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2024-08-27 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Key Research and Development Program of China
|
|
2022YFD2400301
|
|
| National Natural Science Foundation of China (NSFC)
|
|
No.32073002
|
|
| Science and Technology Planning Project of Guangdong Province, China
|
|
2020B1212060058
|
|
|
|
| 提交者 |
Xiaoyuan
Song (songxy5@ustc.edu.cn)
|
| 提交单位 |
University of Science and Technology of China |
| 提交日期 |
2024-06-05 |
