| Accession |
PRJCA027246 |
| Title |
Estrogen oppositely regulates pulmonary hypertension via METTL3/PFKFB3 under normoxia and hypoxia |
| Relevance |
Medical |
| Data types |
Proteomics
|
| Organisms |
Rattus norvegicus
|
| Description |
PASMCs cultured under normoxia and hypoxia for 48 h were collected for the TMT-labeled quantitative proteomics analysis. The analysis was performed using aQ-Exactive mass spectrometer (Thermofisher, USA) by Shanghai Lu-Ming Biotech Co.,Ltd. (Shanghai, China). Briefly, PASMCs were lysed with 300 µL lysis buffer, followed by protein concentration measurement and quality control of the protein samples. Then the lyophilized samples were labeled with TMT. The mass spectrometry analysis using Proteome Discover 2.4 (Thermofisher, USA). |
| Sample scope |
Synthetic |
| Release date |
2024-10-14 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
|
|
Estrogen Oppositely Regulates Pulmonary Hypertension via METTL3/PFKFB3 Under Normoxia and Hypoxia
|
American Journal of Respiratory Cell and Molecular Biology
|
10.1165/rcmb.2024-0042OC
|
2024
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
82171631
|
Research on the mechanism of estrogen reverse regulation of METTL3/PFKFB3 expression in pulmonary arterial smooth muscle cells and the occurrence of pulmonary arterial hypertension under normoxic/hypoxic conditions.
|
|
|
| Submitter |
Xiaosa
Li (xiaosali@gzhmu.edu.cn)
|
| Organization |
Guangzhou Medical University |
| Submission date |
2024-06-20 |
