| 项目编号 |
PRJCA027606 |
| 项目标题 |
Integrating Single-cell RNA and T-cell/B-cell Receptor Sequencing with Mass Cytometry Reveals Dynamic Trajectories of Human Peripheral Immune Cells from Birth to Old Age |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
Single cell sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Comprehensive understanding of the evolution of the immune landscape in humans across the entire lifespan at single-cell transcriptional and protein levels, during development, maturation, and senescence is currently lacking. We recruited a total of 220 healthy volunteers from Shanghai Pudong Aging Cohort (NCT05206643), spanning 13 age groups from 0 to over 90 years, and profiled their peripheral immune cells through single-cell RNA-sequencing coupled with single T-cell/B-cell receptor sequencing, high-throughput mass cytometry, bulk RNA-sequencing, and flow cytometry validation experiments. |
| 样品范围 |
scRNA-seq, bulk RNA-seq, and CyTOF of Human PBMCs |
| 发布日期 |
2024-10-22 |
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82230014
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81930007
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32100426
|
|
| National Science Fund for Distinguished Young Scholars
|
|
81625002
|
|
| Shanghai Municipality Science and Technology Commission
|
|
22JC1402100
|
|
| Shanghai Municipality Science and Technology Commission
|
|
22DZ2292400
|
|
| Shanghai Municipality Science and Technology Commission
|
|
21YF1445000
|
|
| Shanghai Municipal Health Commission
|
|
2022JC013
|
|
| Shanghai Municipal Health Commission
|
|
2023ZZ02021
|
|
| Shanghai Municipal Health Commission
|
|
GWVI-11.1-26
|
|
| Shanghai Municipal Education Commission
|
|
SHSMU-ZDCX20210700
|
|
|
|
| 提交者 |
Jun
Pu (pujun310@hotmail.com)
|
| 提交单位 |
Renji Hospital, School of Medicine, Shanghai Jiao Tong University |
| 提交日期 |
2024-07-01 |
