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Accession PRJCA027750
Title Single-cell RNA sequencing illuminates the ontogeny, conservation, and diversification of cartilaginous and bony fish lymphocytes
Relevance Agricultural
Data types Single cell sequencing
Organisms Chiloscyllium plagiosum
Danio rerio
Cynoglossus semilaevis
Description Elucidating cellular architecture and cell-type evolution across species is central to understanding immune system functioning and susceptibility to disease. BCR-, TCR- and MHC-dependent adaptive immune systems first emerged in a common ancestor of cartilaginous and bony fishes but the evolutionary history of gnathostome immune cell types remains unclear. Here, we systematically analyzed immune system architecture and gene expression signatures by creating an atlas of immune cells of cartilaginous (white-spotted bamboo shark) and bony (zebrafish and Chinese tongue sole) fish. Most cell types correlated well with their counterparts across species. In the bamboo shark, we found a phagocytic function of B cells with specific expression of several pattern recognition receptors and identified a T cell sub-cluster co-expressing both T and B cell markers. In contrast to a division by function in the bony fishes, we show close linkage and poor functional specialization among lymphocytes in the cartilaginous fish. Our cross-species single-cell comparison provides a valuable resource for uncovering the origin and evolution of the gnathostome immune system.
Sample scope Multispecies
Release date 2024-07-05
Grants
Agency program Grant ID Grant title
other 2022YFD2400100 National Key Research and Development Program of China
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences 20603022022002 Central Public-interest Scientific Institution Basal Research Fund
other 2017ASTCP-ES06 the Taishan Scholar Project Fund of Shandong of China, the National Ten-Thousands Talents Special Support Program, the AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology
Chinese Academy of Fishery Sciences 2023TD19 the Central Public-interest Scientific Institution Basal Research
Laoshan Laboratory LSKJ202203802 the Central Public-interest Scientific Institution Basal Research
Submitter Yanan    Li  (nan36@foxmail.com)
Organization Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
Submission date 2024-07-05

Project Data

Resource name Description
BioSample (60)  show -
GSA (1) -
CRA017585 Single-cell RNA sequencing illuminates the ontogeny, conservation, and diversification of cartilaginous and bony fish lymphocytes