| Description |
The 35-day-old Col, cdf2, m1, m2, hpr1 and grp7 mutants were collected to further analysis. The derivatized samples underwent analysis on an Agilent 7890B gas chromatography system paired with an Agilent 5977A MSD system. A DB-5MS fused-silica capillary column was employed for the separation of the derivatives. Helium, with a purity of over 99.999%, served as the carrier gas, flowing through the column at a constant rate of 1 mL/min. The injector temperature was maintained at 260. The injection volume was 1 μL, administered in splitless mode. The initial oven temperature was set at 60 and held for 0.5min, then increased to 125 at a rate of 8/min, further ramped to 210 at 5/min, then to 270 at 10/min, and finally to 305 at 20/min , where it was held for 5 min. The MS quadrupole and ion source (electron impact) temperatures were adjusted to 150 and 230, respectively. The collision energy was set at 70 eV. Mass spectrometric data was collected in full-scan mode (m/z 50-500), and the solvent delay time was specified as 5 min. |
