| 项目编号 |
PRJCA028153 |
| 项目标题 |
Whole genome sequencing analysis of 5NABE off-target sites in HEK293T |
| 涉及领域 |
Medical |
| 数据类型 |
Whole genome sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
To test the off-targeting on the whole genome of 5NABE, whole genome sequencing was applied. Three groups of data were set up, each with three biological replicates. The first group is a negative control without transfection of any plasmid; the second group is a treatment control with transfection of a plasmid only expressing 5NABE protein; and the third group is an experimental group with transfection of plasmids expressing 5NABE and targeting KLF4 (SITE 10) crRNA. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2024-07-19 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39179566
|
Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells
|
Nature Communications
|
10.1038/s41467-024-51695-x
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Chinese Academy of Sciences (CAS)
|
Strategic Priority Research Program of Chinese Academy of Sciences (CAS)
|
XDA24020101
|
|
|
|
| 提交者 |
Hua
Xiang (xiangh@im.ac.cn)
|
| 提交单位 |
Institute of Microbiology, Chinese Academy of Sciences |
| 提交日期 |
2024-07-16 |
