| Accession |
PRJCA028709 |
| Title |
The interactome of Musclin on the membrane of adipocytes |
| Relevance |
Medical |
| Data types |
The mass spectrometry proteomic data
|
| Organisms |
Mus musculus
|
| Description |
A proximity-dependent biotin identification (BioID) assay was used to identify the interactome for Musclin on the plasma membrane of adipocytes. pCMV-BirA* (Control), pCMV-Musclin-BirA* plasmids were transfected into the HEK293T cells. After transfection for 12 h, cell medium-enriched in BirA* or Musclin-BirA* fusion protein were collected for incubation of C3H10T1/2-derived adipocytes, supplemented with 80 μM biotin for 24 h. The membrane-associated proteins from adipocytes were separated and were then pulled down using streptavidin agarose. The agarose beads were pelleted for obtaining Musclin-interacted proteins, followed by denaturation, silver staining and M.S. analysis. |
| Sample scope |
Monoisolate |
| Release date |
2024-09-01 |
| Publication |
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2018YFA0800400
|
|
|
|
| Submitter |
Zhuoxian
Meng (zxmeng@zju.edu.cn)
|
| Organization |
Zhejiang Univeristy |
| Submission date |
2024-07-31 |
