| Accession | PRJCA028724 | ||||||||||||||||
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| Title | Unraveling the Key Role of Chromatin Structure in Cancer Development through Epigenetic Landscape Characterization of Oral Cancer | ||||||||||||||||
| Relevance | Cancer | ||||||||||||||||
| Data types |
Whole genome sequencing
Epigenomics Exome Transcriptome or Gene expression Raw sequence reads Genome sequencing |
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| Organisms | Homo sapiens | ||||||||||||||||
| Description | Epigenetic alterations, such as those in chromatin structure and DNA methylation, have been extensively studied in a number of tumor types. But oral cancer, particularly oral adenocarcinoma, has received far less attention. Here, we combined laser-capture microdissection and muti-omics mini-bulk sequencing to systematically characterize the epigenetic landscape of oral cancer, including chromatin architecture, DNA methylation, H3K27me3 modification, and gene expression. In carcinogenesis, tumor cells exhibit reorganized chromatin spatial structures, including compromised compartment structures and altered gene-gene interaction networks. Notably, some structural alterations are observed in phenotypically non-malignant paracancerous but not in normal cells. We developed transformer models to identify the cancer propensity of individual genome loci, thereby determining the carcinogenic status of each sample. Insights into cancer epigenetic landscapes provide evidence that chromatin reorganization is an important hallmark of oral cancer progression, which is also linked with genomic alterations and DNA methylation reprogramming. In particular, regions of frequent copy number alternations in cancer cells are associated with strong spatial insulation in both cancer and normal samples. Aberrant methylation reprogramming in oral squamous cell carcinomas is closely related to chromatin structure and H3K27me3 signals, which are further influenced by intrinsic sequence properties. Our findings indicate that structural changes are both significant and conserved in two distinct types of oral cancer, closely linked to transcriptomic alterations and cancer development. Notably, the structural changes remain markedly evident in oral adenocarcinoma despite the considerably lower incidence of genomic copy number alterations and lesser extent of methylation alterations compared to squamous cell carcinoma. We expect that the comprehensive analysis of epigenetic reprogramming of different types and subtypes of primary oral tumors can provide additional guidance to the design of novel detection and therapy for oral cancer. | ||||||||||||||||
| Sample scope | Multiisolate | ||||||||||||||||
| Release date | 2024-07-31 | ||||||||||||||||
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| Submitter | Yanyi Huang (yanyi@pku.edu.cn) | ||||||||||||||||
| Organization | Peking University | ||||||||||||||||
| Submission date | 2024-07-31 |
| Resource name | Description |
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| BioSample (102) show | - |