| 项目编号 |
PRJCA028780 |
| 项目标题 |
NatA Complex-mediated Acetylation Promotes the Tolerance of Phytopathogenic Fungus Verticillium dahliae to Biocontrol Bacterium |
| 涉及领域 |
Agricultural |
| 数据类型 |
Metabolomics
|
| 物种名称 |
Verticillium dahliae
|
| 描述信息 |
Rhizosphere microbes play a crucial role in shaping plant growth and disease resistance, while previous research has focused on how plants recruit beneficial microbiomes and how biocontrol agents combat phytopathogens, our understanding of how phytopathogens resist biocontrol bacteria remains limited. Here, we identified that the Bacillus amyloliquefaciens strain TG1-2 demonstrated broad-spectrum antimicrobial activity against various phytopathogens. Further exploration revealed that surfactins are the primary compounds responsible for this antimicrobial effect. A genetic screen in the phytopathogenic fungus Verticillium dahliae showed that the NatA acetyltransferase complex plays a crucial role in promoting fungal tolerance by counteracting TG1-2-induced apoptosis. Dysfunction of the NatA complex increased fungal sensitivity to TG1-2, whereas overexpression exhibited the opposite effect. The acetylation of the molecular chaperone Hsp83 by the NatA complex leads to the formation of a co-chaperone complex Hsp83-Sti1-Hsp70 involved in protein quality control. Disruption of Hsp83 function results in dissociation of the co-chaperon complex and increased apoptosis. Notably, TG1-2 treatment significantly inhibited the Nat A complex-mediated acetylation, enhancing fungal apoptosis. In summary, our study uncovers a novel antagonistic mechanism employed by phytopathogenic fungus to resist biocontrol bacteria, providing new targets and a theoretical foundation for enhancing the effectiveness of biocontrol bacteria in developing biopesticides against phytopathogenic fungi. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-08-02 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40595600
|
Reduced fungal protein acetylation mediates the antimicrobial activity of a rhizosphere bacterium against a phytopathogenic fungus
|
Nature Communications
|
10.1038/s41467-025-60870-7
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
32270200
|
|
|
|
| 提交者 |
Yingchao
Zhang (1037896629@qq.com)
|
| 提交单位 |
CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology |
| 提交日期 |
2024-08-02 |
