Accession

PRJCA029559

Title

CoIP-MS based method to identify FNIP1 binding proteins in exercising muscle

Relevance

Medical

Data types

The MS proteomics data

Organisms

Mus musculus

Description

Using muscle-specific FNIP1 (WT) and FNIP1 (S220A Tg) muscle tissues, we identified approximately 600 potential FNIP1 (WT) or FNIP1 (S220A) binding proteins in exercising muscle tissue of the corresponding mice by the CoIP-MS method, proteomic analysis showed that both FNIP1 (WT) and FNIP1 (S220A) interacted with major mitochondrial proteins, such as the respiratory complex subunits. Notably, FNIP1 (S220A) specifically interacted with additional mitochondrial subunits that were not observed for FNIP1 (WT), including protein involved in NADH metabolism and sulfur cluster binding. These data suggested that non-phosphorylated FNIP1 (S220A) could enhance its interaction with mitochondria.

Sample scope

Multiisolate

Release date

2024-08-28

Publication

PubMed ID	Article title	Journal name	DOI	Year
38324677

Grants

Agency	program	Grant ID
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2018YFA0800700
National Natural Science Foundation of China (NSFC)		91857105
National Natural Science Foundation of China (NSFC)		31922033
Fundamental Research Funds for the Central Universities		021414380511
Fundamental Research Funds for the Central Universities		021414380529
Fundamental Research Funds for the Central Universities		021414380533
Fundamental Research Funds for the Central Universities		021414380524
Shenzhen Science and Technology Program		JCYJ20190807161213744
Shenzhen Science and Technology Program		JCYJ20220530145613030

Submitter

Danxia Zhou (zhoudx@smail.nju.edu.cn)

Organization

Nanjing University

Submission date

2024-08-28

Project Data

Resource name	Description