| Accession |
PRJCA030078 |
| Title |
A novel protein NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in renal tubular epithelial cells-3 |
| Relevance |
Medical |
| Data types |
metabonomics
|
| Organisms |
Homo sapiens
|
| Description |
Proteins immunoprecipitated from SDS-PAGE gels were digested as previously described. The gel pieces were washed with 50% ACN for 30 min and then with 100% ACN for 30 min. Following reduction and alkylation, the proteins were digested with trypsin at 37 °C for 12 h. The supernatant containing the digested peptides was collected and processed for desalination using an HPLC buffer. The samples were analyzed by LC-MS/MS using an Easy-nLC1200 chromatograph (Thermo Scientific) and a Q Exactive plus mass spectrometer (Thermo Scientific). Furthermore, data analysis was performed using the predicted sequence of the NSD1-916aa protein and by searching against the UniProt-reviewed Homo sapiens database with Proteome Discoverer 2.4. |
| Sample scope |
Monoisolate |
| Release date |
2024-09-11 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| the National Natural Science Foundation
|
|
82300820
|
|
| the China Postdoctoral Science Foundation
|
|
2023T160001
|
|
| the China Postdoctoral Science Foundation
|
|
2022M710179
|
|
|
|
| Submitter |
Li
Gao (18056052029@163.com)
|
| Organization |
The First Affiliated Hospital of Anhui Medical University |
| Submission date |
2024-09-11 |
